ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
R. Gunaseelan1 and T. Viswanathan2
1Research and Development Centre, Bharathiar University, Coimbatore – 641 046, India.
2Department of Microbiology, L.R.G Govt. Arts College for Women, Tirupur-641 604, India.
J Pure Appl Microbiol, 2019, 13 (3): 1815-1821 | Article Number: 5621 | © The Author(s). 2019
Received: 18/05/2019 | Accepted: 30/06/2019 | Published: 07/08/2019

Purpose of the research work was to appraise the purified water quality by microbial analysis used in various manufacture in pharmaceutical industry. To have better control water systems samples are collected at regular intervals from the predefined user points. Water samples are checked for Total Aerobic Microbial Count (TAMC), Total Yeast and Mold Count (TYMC), Escherichia coli, Salmonella species, Staphylococcus aureus, Pseudomonas aeruginosa, Out of 2424 samples tested, 93.3% are positive, 6.6% are negative, 0.1% out of alert and no growth observed for TYMC, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Salmonella sp. Investigation performed for 0.1% (4 samples) for which out of alert limit observed and identified the microbes such as Ralstonia solanacearum, Ralstonia mannitolilytica, Bacterium Sanya and Sphingomonas sp. based on the molecular characterization Based on the outcome of the results RO membrane replaced and increased the sanitization frequency. Further water samples were monitored as per the predefined frequency and observed that the results are within acceptance criteria.


Microbial analysis, Purified water, Total Aerobic Microbial Count, Total Yeast and Mold Count, Molecular characterization.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.