ISSN: 0973-7510

E-ISSN: 2581-690X

Fatimah1,2 , Suharjono2, Tri Ardyati2, Ni’matuzahroh1, Afaf Baktir3 and Ahmad Thontowi4
1Department of Biology, Faculty of Science and Technology, University of Airlangga, Indonesia.
2Biology Doctoral Program, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Indonesia.
3Department of Chemistry, Faculty of Science and Technology, University of Airlangga, Indonesia.
4Research Center for Biotechnology, LIPI, Cibinong, Indonesia.
J. Pure Appl. Microbiol., 2016, 10 (1): 151-156
© The Author(s). 2016
Received: 11/11/2015 | Accepted: 09/01/2016 | Published: 31/03/2016
Abstract

The aims of this research were to identify and characterize bacteria isolated from petroleum  contaminated soil, P2(1) isolate. This isolate was able to grow on glucose containing medium and produce biosurfactant. This isolate was identified by morphological, physiological and biochemical characteristics and phylogenetic analysis by 16S rDNA sequences. Morphological characteristics observed were colony and cell morphology. Physiological characteristics were observed by microbact kit 12A, 12B. Phylogenetic tree was constructed by comparing 16S rDNA sequences. The results showed that P2(1) isolate was classified into Genus Arthrobacter based on some characteristics, Gram negative in young culture and Gram positive in old culture, rod coccus cycle, and has cream colony on Nutrient Agar medium. The phylogenetic analysis of 16S rDNA sequences showed that P2(1) isolate has similarity with Arthrobacter sp. XBGRY2 (HQ891968.1) with 95% similarity.  It can be concluded that the isolate was identified as Arthrobacter sp. P2(1) (KU361211).

Keywords

Arthrobacter sp. P2(1), Characteristics of isolate, Phylogenetic analysis, Biosurfactant.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.