Baculovirus is an ideal pathogenic microbial pesticide for the control of pests in agriculture and forestry. This study isolated and purified a new viral strain that can infect the larvae of Neodiprion zhejiangensis Zhou & Xiao, named NezhNPV. Ultrastructure studies indicated that the virus is a single nucleocapsid nucleopolyhedrovirus with a diameter of 0.47-0.91 ìm. Biological activity assays showed that NezhNPV is highly virulent in the third instar larvae of N. zhejiangensis, with an LC50 of 1.15×104 polyhedral inclusion bodies (PIB)/ml. The LT50 of the virus increased with decreasing viral concentrations. When the viral concentrations were 7×107, 7×106, 7×105, 7×104, and 7×103 PIB/ml, the LT50 values were 3.70, 4.12, 4.56, 5.08, and 6.24 d, respectively. Interestingly, unlike the Hymenoptera virus, which only infects the midgut, this virus can infect various tissues of parasites, such as the fat body, trachea, and epidermal cells.
Neodiprion zhejiangensis Zhou & Xiao, Nucleopolyhedrovirus, Biological control, Ultrastructure, Pathology.
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