ISSN: 0973-7510

E-ISSN: 2581-690X

Qinghua Wang, Yuzhu Wang, Ruizhen Wang and Yongan Zhang
The Key Laboratory of Forest Protection, State Forestry Administration of China, Research Institute of Forest Ecology, Environment and [rotection, Chinese Academy of Forestry, Beijing, China.
J. Pure Appl. Microbiol., 2016, 10 (1): 103-108
© The Author(s). 2016
Received: 03/08/2015 | Accepted: 14/09/2015 | Published: 31/03/2016
Abstract

Baculovirus is an ideal pathogenic microbial pesticide for the control of pests in agriculture and forestry. This study isolated and purified a new viral strain that can infect the larvae of Neodiprion zhejiangensis Zhou & Xiao, named NezhNPV. Ultrastructure studies indicated that the virus is a single nucleocapsid nucleopolyhedrovirus with a diameter of 0.47-0.91 ìm. Biological activity assays showed that NezhNPV is highly virulent in the third instar larvae of N. zhejiangensis, with an LC50 of 1.15×104 polyhedral inclusion bodies (PIB)/ml. The LT50 of the virus increased with decreasing viral concentrations. When the viral concentrations were 7×107, 7×106, 7×105, 7×104, and 7×103 PIB/ml, the LT50 values were 3.70, 4.12, 4.56, 5.08, and 6.24 d, respectively. Interestingly, unlike the Hymenoptera virus, which only infects the midgut, this virus can infect various tissues of parasites, such as the fat body, trachea, and epidermal cells.

Keywords

Neodiprion zhejiangensis Zhou & Xiao, Nucleopolyhedrovirus, Biological control, Ultrastructure, Pathology.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.