The process of symbiotic nitrogen fixation results in generation of reactive oxygen species such as superoxide anion (O2–) and hydrogen peroxide (H2O2). The response of Azorhizobium caulinodans to these toxic oxygen species is an important factor in nodulation and nitrogen fixation. It has been found that pretreatment of A.caulinodans with low, non-lethal levels of a selective oxidant viz., H2O2 (200µM) imparts resistance to oxidative mortality. The catalase activity was also found to be higher in H2O2 pre-treated cells than untreated controls.
Azorhizobium caulinodans, Catalase, Hydrogen peroxide
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