The bacterium Pseudomonas stutzeri ML-18 was isolated from the kitchen waste water of a sweet shop. The production of amylase by this bacterium was investigated and optimized using statistical approaches (Response Surface Methodology (RSM); Box–Behnken design). The optimized medium was obtained with these conditions; (% w/v) KH2PO4 0.1, Na2PO4 0.25, NaCl 0.1, KNO3 0.1, CaCl2 0.005, tryptone 0.2, MgSO4.7H2O 0.005, starch 1 and pH 8. The amylase activity in optimized medium was 243.614 U/ml. furthermore, high level of amylase production (245.612 U/ml) was observed by using fermentor system.
Amylase, Pseudomonas, RSM, optimization
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