High Level Amylase Production by Pseudomonas stutzeri ML-18; Isolation, Identification and Media Optimization in a Fermentation System using Response Surface Methodology (RSM)

ISSN: 0973-7510

E-ISSN: 2581-690X

High Level Amylase Production by Pseudomonas stutzeri ML-18; Isolation, Identification and Media Optimization in a Fermentation System using Response Surface Methodology (RSM)

Aboozar Kazemi¹, Mojtaba Lorpour¹, Ahmad Gholami^1,2, Seyed Reza Karimi-Ghavamabadolya^1,2, Azam Safari¹, Kavous Solhjoo³ and Younes Ghasemi^1,2

¹Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

²Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.

³Department of Microbiology, Jahrom University of Medical Sciences, Jahrom, Iran.

J Pure Appl Microbiol. 2015;9(Spl. Edn. 2):127-133

Received: 20/01/2014 | Accepted: 10/02/2014 | Published: 30/11/2015

Abstract

The bacterium Pseudomonas stutzeri ML-18 was isolated from the kitchen waste water of a sweet shop. The production of amylase by this bacterium was investigated and optimized using statistical approaches (Response Surface Methodology (RSM); Box–Behnken design). The optimized medium was obtained with these conditions; (% w/v) KH₂PO₄ 0.1, Na₂PO₄ 0.25, NaCl 0.1, KNO₃ 0.1, CaCl₂ 0.005, tryptone 0.2, MgSO₄.7H₂O 0.005, starch 1 and pH 8. The amylase activity in optimized medium was 243.614 U/ml. furthermore, high level of amylase production (245.612 U/ml) was observed by using fermentor system.

Keywords

Amylase, Pseudomonas, RSM, optimization

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