ISSN: 0973-7510

E-ISSN: 2581-690X

Jie-Ming Qin, Dang-Quan Zhang , Fang-Yuan Wu, Han-Jie He, Rong Chen and Xiao-Lei Zhang
1Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees (Ministry of Education) / Hunan Provincial Key Laboratory of Forestry Biotechnology / Cooperative Innovation Center of Cultivation and Utilization for Non-Wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha 410004, China.
J Pure Appl Microbiol. 2015;9(3):2069-2076
© The Author(s). 2015
Received: 28/04/2015 | Accepted: 03/07/2015 | Published: 30/09/2015
Abstract

Dendrobium officinale was widely used as a rising valuable forest Chinese medicinal herb in recent years in China. By the systematical researches on seed aseptic germination, protocorm induction and proliferation, rooting and hardening-off culture, seedling transplanting and tending, the highly efficient regeneration system and domestication transplanting technology for D. officinale were established. The results showed that 1/2 MS without any phytohormone is the optimal medium for seed aseptic germination of D. officinale, the medium suitable for protocorm induction and proliferation is MS + 1.0 mg/L 6-BA (6-Benzylaminopurine) +1.0 mg/L NAA (1-Naphthaleneacetic acid) +1.0 mg/L KT (kinetin) according to the orthogonal tests, the optimal medium for rooting and hardening-off culture of D. officinale is 1/2 MS+ 0. 2mg /L IBA (3-indolebutyric acid) + 0.5% activated carbon + 40% potato extract, and the corresponding seedling transplanting medium is the 1:1:5 combination of vermiculite, perlite and humus soil. The high-efficient regeneration and transplanting technique obtained will contribute to the industrialized seedling production of Biomedical D. officinale.

Keywords

Dendrobium officinale, Regeneration system, Seedling transplanting, Medicinal herb, Biomedicine

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