Herbicide Induced Reversion to Prototrophy of a Non-Sporulating Mutant of Anabaena doliolum

ISSN: 0973-7510

E-ISSN: 2581-690X

Herbicide Induced Reversion to Prototrophy of a Non-Sporulating Mutant of Anabaena doliolum

Anupma Srivastava¹ and A.K. Pandey²

¹Department of Botany, Shri A.K.A.P.G. College, Varanasi, India.

²Biological Research Lab, Department of Botany and Biotechnology, Kutir P.G. College, Chakkey – 222 146, Jaunpur, India.

J Pure Appl Microbiol. 2009;3(2):731-734

Received: 10/04/2009 | Accepted: 23/07/2009| Published: 31/10/2009

Abstract

Spontaneous and MNNG-induced reversion of non-sporulating mutant
(Ad-Ns, het⁺ nif⁺ sp^–) of the cyanobacterium A. doliolum, to prototrophy (Ad-S,het⁺ nif⁺ sport⁺) was investigated to compare the mutagenic potential of the herbicide alachlor. Alachlor induced reversion to protrophy was greater than spontaneous and MNNG-induced frequencies. The prototrophic revertants, either spontaneous or MNNG and alachlor induced, of non-sporulating mutants have a heterocyst frequency of 5.1±0.6% as in parent. Sporulation started during stationary phase of the growth of the revertants (Ad-S, het⁺ nif⁺ sport⁺) and spore frequency reached upto 92.0±0.25% against parents frequency (100%) the growth pattern of the revertant was slightly and significantly slower than the parent in N2-medium.

Keywords

Cyanobacterium, Revertants, Prototrophy, Heterocyst, Sporulation, Herbicide

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