ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Mohd. Imran1, Iqbal Ahmad1, Tharcisse Barasubiye2, Hussein H. Abulreesh3,4 , Samreen1, Mohammad Khalil Monjed3,4 and Khaled Elbanna3,4,5
1Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, India.
2Agriculture and Agri-Food Canada, 960 Carling Ave., Ottawa, Ontario, KIA 0C6 Canada.
3Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia.
4Research Laboratories Center, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, Saudi Arabia.
5Department of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum, Egypt.
J. Pure Appl. Microbiol., 2020, 14 (1): 157-170 | Article Number: 6027 | © The Author(s). 2020
Received: 22/12/2019 | Accepted: 23/03/2020 | Published: 02/04/2020

In this study fungal profiles of agricultural field soil irrigated with industrial wastewater and sewage containing varying concentrations of heavy metals (Chromium, Nickel, Cobalt, Copper and Cadmium) have been investigated. The impact of long term heavy metal contamination on emergence of heavy metal tolerant soil fungal population, changes in morphological diversity and metal tolerance limits among isolated fungi was studied. The agricultural field soil received long term (>20 years) wastewater application showed metal accumulation compared to the untreated soil. The viable count of soil fungal population from three different agricultural field soil was found in order of 105 to 10 4 CFU gm-1 of soil indicating a normal viable count with little variations. Viable plate count of fungal population on metal amended plates decreased with increasing concentration of tested metals (Cr6+, Cd++.  Cu++, Co++ and Ni++) from 100 to 400 µgml-1. The decrease was higher on cadmium amended plates and lower against Chromium.  The control site, which did not receive wastewater application showed relatively less metal tolerant fungal viable count on Cd++ and Ni++  plates when compared at 100 µgml-1as compared to contaminated sites. Similarly, presence of metal tolerant fungal population was also observed from wastewater sample.  The common soil fungi isolated and characterized from metal amended plates belong to 18 genera and 15 unidentified species. Occurrence of different fungal genera from site A B and C indicated different patterns of decrease on different metal amended plates with increasing concentration. Among these 73 isolated fungal species  a high level of tolerance was recorded to Cr6+ followed by Cu++, Co++ and Ni++ while the lowest level of tolerance was for Cd++. The minimum inhibitory concentration (MIC) values of 73 metal tolerant fungal isolates, was ranged from 200 to 2000 μgml-1 against one or more heavy metals. The level of tolerance to heavy metals also varied even among the isolates of single genus. Aspergillus was the predominant genus recovered from contaminated soils where the MIC values are highly varied among different isolates of Aspergillus. The current study found that long term release of wastewater has not disturbed the fungal population dynamics in contaminated sites as compared to uncontaminated sites. However, it tends to exert selective pressure on fungal populations of soil, leading to the development of increased level of metal tolerance in fungal species.


Aspergillus, heavy metals, soil fungi, metal tolerance, MIC, wastewater.

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.