ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Manjari Barsainya and Devendra Pratap Singh
Department of Environmental Science, Babasaheb Bhimrao Ambedkar University,  Vidya Vihar, Raibareily Road, Lucknow – 226 025, India.
J. Pure Appl. Microbiol., 2018, 12 (4): 2123-2134 | Article Number: 5262
Received: 10/08/2018| Accepted: 16/10/2018 |Published:
Abstract

The present work highlights a low-cost, ecofriendly and simple procedure for the synthesis of zinc oxide nanoparticles (ZnONPs), mediated by extracellular secondary metabolite pyoverdine – a siderophore produced by Pseudomonas aeruginosa. The in vitro bio-reduction of zinc nitrate into ZnONPs in the presence of siderophore pyoverdine was ascertained by UV–visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy and transmission electron microscopy (TEM) analysis. The synthesized ZnONPs were distinguished by a characteristic absorption peak at 416 nm in the UV–visible range. The TEM pictures of ZnONPs showed pseudospherical morphology, with particle size ranging between 50 and 100 nm. X-ray diffraction peaks obtained at 31.73°, 34.37°, 36.24°, 47.40°, 56.68°, 63.0° and 67.8° corresponding to the lattice plane of [100], [002], [101], [102], [110], [103] and [112] suggested the polycrystalline wurtzite structure of the nanoparticle. The ZnONPs were found to have an antibacterial effect against Gram-positive bacteria (Staphylococcus aureus and Bacillus sp.) and Gram-negative bacteria (Escherichia coli), and also showed a fungicidal effect against phytopathogenic fungi such as Rhizoctonia solani, Fusarium sp. and Penicillium sp. The present approach of using exogenously produced bacterial siderophore offers a unique in vitro green method of synthesizing ZnONPs, where a direct contact between the toxic metal and biological resource material can be avoided.

Keywords

Pseudomonas aeruginosa, siderophore, XRD, FTIR, TEM, zinc oxide nanoparticles.

Article Metrics

Article View: 770

Share This Article

© The Author(s) 2018. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.