ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Bennet Rohan D.1, Raji P.1, Divya Kumar M.1, Ratna Geethika Gandham1, Kripu Sharma V.1, Keerthana D.1, Karishma S.1, Antony V. Samrot2 , Iyappan Petchi2, Thirumurugan R.3, Sajna Keeyari Purayil2, Paulraj Ponnaiah2, Jenifer Selvarani A.1 and Prakash P.1
1Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai – 600 119, Tamil Nadu, India.
2Department of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, MAHSA University, Jalan SP2, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia.
3Department of Transfusion Medicine, JIPMER, Puducherry – 605 006, India.
J. Pure Appl. Microbiol., 2020, 14 (1): 301-306 | Article Number: 5970
https://doi.org/10.22207/JPAM.14.1.31 | © The Author(s). 2020
Received: 28/11/2020 | Accepted: 03/03/2020 | Published: 28/03/2020
Abstract

The aqueous extracts of Euphorbia hirta was used to synthesise silver nanoparticles using bioreduction method. The nanoparticles were characterised by UV Vis spectroscopic analysis, SEM, EDX, AFM, XRD analysis. The silver nanoparticles were also tested for antibacterial activity in Pseudomonas aeruginosa and Bacillus subtilis. The minimum inhibitory concentration for the synthesised nano particles were also tested against the two bacterial species for the least concentration of 0.5µg/mL. The swarming motility assay and protein leakage assay was also tested for the nanoparticle. The silver nanoparticles were found to be much effective.

Keywords

Silver nanoparticles, Antibacterial activity, MIC, Protein leakage assay, Euphorbia hirta.

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