ISSN: 0973-7510

E-ISSN: 2581-690X

Dheeraj Pal1 , Nongthombam Boby1, Gurpreet Kaur2, Syed Atif Ali2, Satish Kumar1 and Pallab Chaudhuri2
1Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar – 243122, India.
2Division of Bacteriology, Indian Veterinary Research Institute, Izatnagar – 243122, India.
J. Pure Appl. Microbiol., 2016, 10 (3): 2449-2452
© The Author(s). 2016
Received: 10/08/2016 | Accepted: 01/09/2016 | Published: 30/09/2016
Abstract

Gold nanoparticles (AuNPs) plasmon changes, as a result of additives and their follow up reaction in solution around zeta space of AuNPs, have been extensively used for analyte detection. Biomolecules like protein or nucleic acid, in conjugated or non-conjugated forms with AuNPs, when allowed to react with complimentary molecule such as antibody or nucleic acid target, lead to visual colour changes and may offer diagnostic test. Using synthetic nucleic acid probes, specific to complimentary target gene in a microbe, conjugated probe-AuNPs were exploiting Au-S bond chemistry. Thiolated nucleotide probes were successfully conjugated on AuNPs to prepare gold reagents that reacted specifically with complimentary gene target and not with non-complimentary sequence, the later failed to stabilise the probe-AuNPs and gave red to blue colour changes in solution after addition of salt. This rapid colour change of probe-AuNPs solution is able to differentiate the presence of complimentary and non-complimentary gene target in test solution, thus offer a strategy for detection of microbial genes.

Keywords

Gold nanoparticle, Visual detection, Brucella, Surface Plasmon Resonance.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.