ISSN: 0973-7510
E-ISSN: 2581-690X
In this study, the thermotolerant and high glycerol-producing Saccharomyces cerevisiae strain F3/24 was obtained after three rounds of genome shuffling of UV and NTG mutants derived from the original strain Saccharomyces cerevisiae NAU-ZH-GY1. The recombinant S. cerevisiae strain F3/24 could grow well at 45 °C. At temperature higher than 39°C, recombinant strain F3/24 always gave higher cell growth and glycerol production. In fermentation medium containing 200g/l glucose and 5g/l corn steep liguor powder, glycerol yield of the shuffled strain F3/24 was 75.3, 72.4, 70.5 and 68.7g/l at 32 ºC, 39 ºC, 42 ºC and 45 ºC for 64h, respectively.
Genome shuffling, Saccharomyces cerevisiae, Glycerol, Thermotolerance
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