Genetic Variation of Antigen B among Echinococcus granulosus Isolates in Tabriz, East Azerbaijan, North West of Iran

ISSN: 0973-7510

E-ISSN: 2581-690X

Genetic Variation of Antigen B among Echinococcus granulosus Isolates in Tabriz, East Azerbaijan, North West of Iran

Abbas Shahbazi¹, Nahid Mazhari², Ardavan Ghazanchaei², Majid Khanmohammadi³ and Esmaeil Fallah²

¹Tabriz Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran.

²Department of Medical Parasitology and Mycology, Tabriz University of Medical Sciences, Tabriz, Iran.

³Department of Laboratory Sciences, Marand Branch, Islamic Azad University, Marand, Iran.

J Pure Appl Microbiol. 2014;8(3):2229-2233

Received: 08/03/2013 | Accepted: 26/05/2013 | Published: 31/06/2014

Abstract

The aim of the present study is to find the polymorphism of the Antigen B among Human and animal isolates in Tabriz, East Azerbaijan, North-West of Iran. 10 hepatic and pulmonary human hydatid cysts and 40 animal hydatid isolates of liver and lung cysts (20 sheep, 20 cattle) were prepared. All analysed isolates within two clusters (human and sheep) gave similar patterns of PCR-RFLP after digestion with AluI (approximately 120 bp and 270 bp) and no digestion occur after incubation with EcoRI. The results of RFLP pattern showed high degree of genetic likeness between human and sheep isolates. Amplification to the DNA samples extracted from germinal layer of cattle isolates not completed by using Eg AgB2 specific primer suggesting suggesting substantial level of inter-strain variation in AgB2 related genes.

Keywords

Echinococcus granulosus, Cystic Hydatid, PCR, Iran, Tabriz

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