This study was conducted to determine the extent of genetic diversity using pulsed-field gel electrophoresis (PFGE) of Salmonella isolates recovered from a variety of sources in Mexico. A total of 54 isolates of Salmonella enterica isolated from a Bell pepper production system, the animal environment (poultry and swine), meat (bovine and chicken), humans (stools and blood), and from unknown sources were examined. The isolates belonged to Salmonella enterica subspecies enterica serotypes Enteritidis, Typhimurium, Choleraesuis, Gallinarum, Newport, and Typhi, and Salmonella enterica subspecies arizonae. Restriction analysis of the 54 isolates with XbaI yielded 30 pulsotypes. S. enterica serotype Enteritidis isolates yielded 16 pulsotypes. Isolates of S. enterica serotype Typhimurium yielded 9 pulsotypes, and the remaining serotypes each yielded one pulsotype. Strains obtained from poultry showed more variation in their PFGE patterns and belonged to serotypes Enteritidis, Typhimurium, and Gallinarum. Serotypes Enteritidis and Typhimurium, were recovered from ca. 52% of samples collected at the field and during packing at a Bell pepper production system that did not apply Good Agricultural Practices. Thus, this study demonstrated that PFGE potentially has the ability to discriminate among Salmonella isolates from different sources since most isolates recovered were grouped based on their origin.