Plant growth promoting Pseudomonas fluorescent siderophore producing rizobacteria were isolated from the rhizosphere and non-rhizospheric soil of cave, forest, fellow land and agriculture field in Chhattisgarh region. Pseudomonas fluorescent single colony was successfully isolated in King’s B media under 260 nm wavelength and forty five yellow green isolates were selected for diversity analysis. The genetic diversity analysis was done by 20 RAPD markers. Out of these 5 primers showed clearly scorablebanding pattern. The selected isolates were distributed into two major clusters. RF9 isolates showed most diverged than others. The similarity coefficient of major clusters ranged from 0.02 to 0.91. The objective of this study was to determine a possible correlation between molecular diversity within Pseudomonas fluorescens isolates of Chhattisgarh.
Pseudomonas fluorescent, Genetic fingerprinting, Diversity assessment, RAPD
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