ISSN: 0973-7510

E-ISSN: 2581-690X

Lincoln Mandal and A.S. Kotasthane
Department of Plant Molecular Biology and Biotechnology, College of Agriculture,
Indira Gandhi KrishiVishwavidyalaya, Raipur – 492 012, India.
J Pure Appl Microbiol. 2012;6(3):1481-1484
© The Author(s). 2012
Received: 15/01/2012 | Accepted: 27/02/2012 | Published: 30/09/2012
Abstract

Plant growth promoting Pseudomonas fluorescent siderophore producing rizobacteria were isolated from the rhizosphere and non-rhizospheric soil of cave, forest, fellow land and agriculture field in Chhattisgarh region. Pseudomonas fluorescent single colony was successfully isolated in King’s B media under 260 nm wavelength and forty five yellow green isolates were selected for diversity analysis. The genetic diversity analysis was done by 20 RAPD markers. Out of these 5 primers showed clearly scorablebanding pattern. The selected isolates were distributed into two major clusters. RF9 isolates showed most diverged than others. The similarity coefficient of major clusters ranged from 0.02 to 0.91. The objective of this study was to determine a possible correlation between molecular diversity within Pseudomonas fluorescens isolates of Chhattisgarh.

Keywords

Pseudomonas fluorescent, Genetic fingerprinting, Diversity assessment, RAPD

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