ISSN: 0973-7510

E-ISSN: 2581-690X

Vahid Rahnamaye Hayati Hagh1,2, Mahmoud Mahami Oskouei1,2 , Ahad Bazmani1 , Abolfazl Miahipour1,2 and Nasrin Mirsamadi3
1Tabriz Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2Department of Parasitology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
3Central Laboratory, Tabriz University of Medical Sciences, Tabriz, Iran.
J Pure Appl Microbiol. 2014;8(5):3995-3999
© The Author(s). 2014
Received: 17/05/2014 | Accepted: 05/07/2014 | Published: 31/10/2014
Abstract

Enterobius vermicularis is one of the most prevalent parasitic helminths, particularly in children. According to the studies, three different genotypes (A, B and C) were revealed from human and chimpanzee. This study conducted to investigate the existence and distribution of different E. vermicularis genotypes based on mitochondrial cytochrome c oxidase subunit 1 (cox1) in northwest of Iran. 45 positive scotch tape samples of E. vermicularis were collected from various areas of Tabriz. After DNA extraction, the targeted DNA region was amplified for the mitochondrial cytochrome c oxidase 1(cox1) gene by nested PCR method. All amplicons were sequenced and then analyzed by specific phylogenetic software. Results of the present study showed that, Enterobius vermicularis have two subtypes including B1 and B2 in northwest of Iran by Cox1 gene sequencing method. In conclusion, B1 and B2 subtypes of Enterobius vermicularis in human are the predominant genotype of this nematode in northwest of Iran. Regarding to the prevalence and public health importance of the disease and role of the parasite genotyping in prevention, control and treatment, further studies are needed to determine genotypes of E. vermicularis in other regions.

Keywords

Enterobius vermicularis, Cytochrome c oxidase 1 (cox1), Genetic classification, Iran

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.