ISSN: 0973-7510

E-ISSN: 2581-690X

Minhaj Arshiya, A.R. Suryawanshi, D.R. More and M.M.V. Baig
Department of Botany and Biotechnolgy, L.B.S. Mahavidyalaya, Dharmabad, Nanded – 431 602, India
*Department of Botany and Department of Biotechnology, Yeshwant Mahavidyalaya, Nanded – 431 602, India.
J Pure Appl Microbiol. 2012;6(3):1219-1226
© The Author(s). 2012
Received: 26/06/2012 | Accepted: 04/08/2012 | Published: 30/09/2012
Abstract

The present study is based on biochemical characterization, pathogenicity test and DNA based technique. Here apart from biochemical test, RAPD analysis was used to distinguish and differentiate among 24 strains of Xac isolated from Marathwada region in Maharashtra. 200 strains of Xac isolated from Citrus lemona from different location within Marathwada region of Maharashtra  and 24 strains representing all the 8 districts were used in this study. All these strains were purified and maintained on YDC (Yeast extract -1gm, D-glucose -2gm, Calcium carbonate -2gm) slants. The presently used primer could resolve genetic diversity and geographical distribution in the study of Xanthomonas axonopodis pv citri and RAPD marker gave reproducible and reliable results.

Keywords

Xac Strains, DNA based techinques, Citrus Canker

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