ISSN: 0973-7510

E-ISSN: 2581-690X

S.K. Samal, B.R. Samantaray and B.K. Das
Central Institute of Freshwater Aquaculture, P.O. Kausalyaganga, Bhubaneswar – 751 002, India,
J Pure Appl Microbiol. 2008;2(1):239-244
© The Author(s). 2008
Received: 21/11/2007 | Accepted: 27/12/2007 | Published: 30/04/2008
Abstract

RAPD profiling of Aeromonas hydrophila MTCC 646 strain was performed in order to analyze the possible genetic variability in this bacterium, the causative agent of epizootic ulcerative syndrome and hemorrhagic septicaemia. Out of one hundred sixty random decamer primers of OPA, OPB, OPC, OPE, OPF, OPJ, OPK and OPY screened against genomic DNA of A. hydrophila, sixty four amplified good, consistent and reproducible fingerprints. The greatest number of fragments was found with the use of primers OPE 15 (8 bands) and OPK 07 (9 bands). The molecular weight of the amplified products of A. hydrophila MTCC 646 against all the tested primers ranged from 0.22 kb to 3.4 kb. The clear, distinct and reproducible DNA fragments of selected primers can be used as standard for identifying new strains of A. hydrophila.

Keywords

Aeromonas hydrophila, RAPD-PCR, Genomic DNA, Primer

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