Extracts of Evolvulus alsinoides L. leaves were investigated for their radical scavenging activity using two different in vitro assays, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The methanolic extract possessed the highest radical scavenging activity in both assays, DPPH (IC50-120 µg/ml ) and ABTS (IC50-100 µg/ml). Total phenolic content was also determined using Folin–Ciocalteu reagent. The highest total phenolic content was detected in the methanol and ethyl acetate extracts (16.8mg/g GAE and 15.6mg/g GAE, respectively). A correlation between radical scavenging capacities of samples with total phenolic compound content was observed. GC-MS analysis of methanolic extract showed that the Bicyclo 5.1.0 octane (7.02%), Hexadecanoic acid (5.75%), Heptadecanoic acid (5.58%) were the major constituents.
Evolvulus alsinoides L., DPPH, ABTS, Phenolics, GC-MS analysis
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