Fingerprinting of Cellulose Degrading Bacteria from Agricultural Soils

ISSN: 0973-7510

E-ISSN: 2581-690X

Machiavelli^2,3, N. Tejoprakash² and Sunil Khanna^1,2

¹Department of Biotechnology and Bioinformatics, NIIT Institute of Information Technology (TNI), Balaji Estate, Kalkaji New Delhi – 110 019, India.

²Department of Biotechnology & Environmental Sciences, Thapar University, Patiala – 147 004, India.

³Department of Biotechnology Engineering, Ambala College of Engineering and Applied Research, Mithapur, VPO: Sambalakha, Ambala – 133 101, India.

J Pure Appl Microbiol. 2009;3(1):143-150

Received: 10/09/2008 | Accepted: 25/10/2008 | Published: 30/04/2009

Abstract

Bacteria were isolated and screened for cellulase activities in agricultural soil of Patiala (India). Three most efficient cellulose degraders identified using 16S rDNA, as Serratia sp., Pseudomonas sp. and Serratia marcescens showed cellulase activity of 3.83, 4.21 and 4.52 mM glucose ml^-1 h^-1respectively. . Enterobacteriaceae Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) was performed to obtain fingerprint and showed clear genetic variability among all three isolates. The bacteria were mass cultured and re-inoculated in soils to study the population dynamics under chickpea (Cicer arietinum L.) cultivation. The role of cellulose degrading bacteria was studied in sustainability of soil and organic carbon indicating good survival of 40.2, 34.4 and 56.8 % of the introduced strains respectively. The highly positive correlation (R²=0.916) among the soil cellulase activity and soil organic carbon was observed during the chickpea plot trails towards inoculated bacterial strains.

Keywords

Agricultural soils, Chickpea, Cellulase, Pseudomonas, Serratia, ERIC-PCR

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