A field experiment was conducted to evaluate the response of L-amino acid micro-mineral complex (AAMMC) on soybean Rhizobium symbiosis at instructional cum research farm, Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.) with six treatments of different combinations (T1-T6) including control. Data revealed that treatment with recommended  dose of fertilizers showed highest value among all the parameters followed by T3 i.e. foliar application of AAMMC @1L/ha 1^st spray at 20-25 DAS and 2^nd spray at 3-4 days before flower initiation. This treatment has highest number of effective nodules (54.5/plant), total N uptake (192.27 kg/ha) and grain yield (25.68 q/ha). However, maximum bacterial population (5×10⁴ per g soil) and rhizobial population (4.59×10⁴ per g soil) were observed in case of T4 i.e. foliar application of L-amino acid micro-mineral complex @1L/ha 1^st spray at 20-25 DAS and 2^nd spray at 3-4 days before flower initiation. The treatment T4 also showed maximum dehydrogenase activity (61 µg TPF/h/g soil) at 50% flowering stage which might be due to the highest microbial population.