Application of Pseudomonas spp.in combination with chitosan reduced the severity of ToLCV diseased plants by 48% at 45 DAI. Application of chitosan or the bacterial inoculant alone was not very effective. The plants inoculated with the combination of Pseudomonas sp. 206(4) and Chitosan recorded the highest phenol content, peroxidase, polyphenol oxidase, chitinase and PALase activities. This treatment also recorded maximum plant height, biomass and chlorophyll content, number of fruits per plant, fruit yield and shelf life of fruits over the diseased control. The semi quantitative PCR analysis revealed the lowest viral load accumulation in plants inoculated with 206(4) and Chitosan. Thus, the study has indicated that the application of Pseudomonas sp. 206(4) and Chitosan effectively reduced the disease severity of ToLCV through ISR as evidenced by lower viral titre and higher production of defense molecules.
Pseudomonas spp., ToLCV, tomato, Chitosan, biocontrol, Bemisia tabaci
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