Field Evaluation of Chitosan and Pseudomonas Sp. on the Biological Control of Tomato Leaf Curl Virus (TolCV) in Tomato

ISSN: 0973-7510

E-ISSN: 2581-690X

Field Evaluation of Chitosan and Pseudomonas Sp. on the Biological Control of Tomato Leaf Curl Virus (TolCV) in Tomato

Shefali Mishra¹, K.S. Jagadeesh¹, P.U. Krishnaraj², A.S. Byadagi³ and A.S. Vastrad⁴

¹Department of Agricultural Microbiology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad – 580 005, India.

²Department of Biotechnology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad – 580 005, India.

³Department of Plant Pathology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad – 580 005, India.

⁴Department of Entomology, College of Agriculture, Dharwad, University of Agricultural Sciences, Dharwad – 580 005, India.

J Pure Appl Microbiol. 2014;8(1):681-688

Received: 18/02/2013 | Accepted: 21/04/2013 | Published: 28/02/2014

Abstract

Application of Pseudomonas spp.in combination with chitosan reduced the severity of ToLCV diseased plants by 48% at 45 DAI. Application of chitosan or the bacterial inoculant alone was not very effective. The plants inoculated with the combination of Pseudomonas sp. 206(4) and Chitosan recorded the highest phenol content, peroxidase, polyphenol oxidase, chitinase and PALase activities. This treatment also recorded maximum plant height, biomass and chlorophyll content, number of fruits per plant, fruit yield and shelf life of fruits over the diseased control. The semi quantitative PCR analysis revealed the lowest viral load accumulation in plants inoculated with 206(4) and Chitosan. Thus, the study has indicated that the application of Pseudomonas sp. 206(4) and Chitosan effectively reduced the disease severity of ToLCV through ISR as evidenced by lower viral titre and higher production of defense molecules.

Keywords

Pseudomonas spp., ToLCV, tomato, Chitosan, biocontrol, Bemisia tabaci

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