DNA isolation preludes any nucleic acid amplification method but also tend to increase the time duration depending on the procedure adapted. The current work is aimed at swotting simplistic methods involving mechanical shearing, boiling and alkaline lysis for bacterial DNA extraction. Comparative analysis of the DNA yield from bacterium “picked” from three different mediums were also tried to select the optimal practice for productive results. Apart from being an expeditious step, Boiling lysis (BL)was also proved to be efficacious for further analysis using PCR-RFLP.