ISSN: 0973-7510

E-ISSN: 2581-690X

Mohamed M. Ibrahim1,2 , Gehan A. El-Gaaly1, Ashraf M. Hatmla1 and Mohamed S. El-Shickc1
1Botany and Microbiology Department, Science College, King Saud University, 11451-P.O. Box 2455, Riyadh, KSA.
2Botany and Microbiology department, Faculty of Science, Alexandria University,
P. O. Box 21511 Alexandria, Egypt.
J Pure Appl Microbiol. 2014;8(1):817-824
© The Author(s). 2014
Received: 11/11/2013 | Accepted: 09/01/2014 | Published: 28/02/2014
Abstract

Currently, there is a growing need to develop environmentally benign nanoparticles synthesis processes that do not use toxic chemicals in the synthesis protocol. The present study reports an environmentally friendly method for synthesis of silver nanoparticles from two seaweeds named Cladophora nitellopsis and Sargassum latifllium.  Ag-NPs bactericidal impact on Bacillus subtilis and Escherichia coli. Scanning and Transmission electron microscopy (SEM &TEM) were used to illustrate thesize and shape of the obtained Ag-NPs. Energy dispersive X-ray (EDX) spectrometer established the existence of elemental sign of the silver and homogenous allocation of silver nanoparticles. Diffraction by using X ray (XRD) analysis for the formed AgNPs revealed spherical plus cubical shapes structure with different planes ranged between 111 to 311 planes. Further, these Ag-nanoparticles show effective antibacterial activity against human pathogens, Bacillus subtilis and Escherichia coli due to high surface to volume ratio. Our work showed a rapid, eco-safety and suitable method for the synthesis of AgNPs by using Cladophora nitellopsis and Sargassum latifllium seaweeds. Therefore, they can be used in pharmaceutical and other biomedical applications.

Keywords

Nanoparticles, TEM, EDX, Green biotechnology, Chladophra, Sargassum

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.