ISSN: 0973-7510

E-ISSN: 2581-690X

Amir Hossein Abadi1, Azad Khaledi2, Abbas Bahador3, Mehdi Mahdavi4 and Davoud Esmaeili1
1Applied Microbiology Research center, and Microbiology Department, Baqiyatallah University of Medical Sciences, Tehran, Iran.
2Antimicrobial Resistance Research Center, Avicenna Research Institute, Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
4Mahdavi, Mehdi, Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.
J. Pure Appl. Microbiol., 2016, 10 (3): 2461-2466
© The Author(s). 2016
Received: 21/03/2016 | Accepted: 27/04/2016 | Published: 30/09/2016
Abstract

Brucella can causes brucellosis in humans and animals, the Most significance of this bacterium is due to its use in biologic wars and bioterrorism.  Vaccination strategy has played a high role in decreasing Brucella infections in many countries of the world. So, this study aimed to investigate the expression, purification and characterization of the mixed Total -OMP- CagA from Brucella abortus and H. pylori as vaccine candidate. After bioinformatics designing by suitable softwares, OMPs proteins of B. abortus extracted, and recombinant protein CagA that cloned in Pet28a, previously, transformed to the E. coli BL21 as expression host.  Then recombinant protein purified by nickel column. Then, CagA and OMPs proteins confirmed with SDS-PAGE and western blotting. In this study construct of Total OMP-CagA was synthesized. OMPs proteins with sizes 25-27 and 36-38 kDa were extracted.  Protein 32 KDa of CagA with concentration 700µg/ml expressed and purified, successfully. In this study, the recombinant protein CagA of H. pylori successfully expressed and total OMPs of B. abortus were successfully extracted and combined together to construct a vaccine candidate. But, complementary studies are required to evaluate the immunological features of mixed OMPs- CagA as novel and efficient vaccine candidate against H. pylori.

Keywords

Brucella. abortus, OMP- CagA, Vaccine candidate.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.