The authors describe the updates on expression of cytochrome P450 genes of fungi (mold and yeast) at mRNA level aiming to facilitate biotechnological application of these versatile biocatalysts in the industry for a series of applications like drug discovery, xenobiotic detoxification and/or bioremediation. This mini-review highlights how to solve the problems that may arise during studying expression profiles of P450s in liquid media, being the crucial step for stepping forward with the genes. Present knowledge on steps to be cared while conducting experiments for P450 clone library construction are summarized. Also, experimental evidences on expression profile of P450 genes in yeast using liquid media have been focused.
Cytochrome P450, Transcriptome, Liquid culture medium, mRNA, yeast, mold
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