ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Ziyaul Haque1, Mohammed Shariq Iqbal1 , Ausaf Ahmad1, Mohd Sajid Khan2, Satarudra Prakash Singh3 and Jyoti Prakash1

1Amity Institute of Biotechnology, Amity University, Lucknow Campus, Lucknow -226 028, Uttar Pradesh, India.
2Department of Biosciences, Integral University, Lucknow – 226 021, Uttar Pradesh, India.
3Mahatma Gandhi Central University, Motihari – 845 401, Bihar, India.
J. Pure Appl. Microbiol., 2020, 14 (1): 327-339 | Article Number: 6081 | © The Author(s). 2020
Received: 10/02/2020 | Accepted: 09/03/2020 | Published: 20/03/2020

The present study was focused on microscopic and molecular characterization with growth optimization of two fungal isolates of Trichoderma spp. (TS) along with their antagonistic activities against Colletotrichum falcatum (CF) and plant growth promoter activity. The microscopic and molecular analysis (Internal transcribed spacer – ITS1 region of 18S rRNA) of these isolates revealed that the isolates were Trichoderma asperellum (MK937669) (TA) and Trichoderma viride (MK503705) (TV). The parameters for maximum growth were found to be in TA and TV in potato dextrose agar (PDA) and Sabouraud dextrose agar (SDA) for media components, 25˚C for temperature and for pH (TA 5.5 and TV 6.5) respectively. The antagonistic activity of TS was studied and it was found to be notable against CF (CF02 strain) with 77.666±4.509% and 72.666±7.094 % of inhibition by TA and TV respectively. As a plant growth promoter it was found that maximum Indole-3-acetic acid (IAA) production with 0.5% of L-tryptophan was with 120.308±5.256µg/mL by TA and 130.101±8.995µg/mL by TV. However, when phosphate solubilization (PS) was studied, it was found maximum at day 5 with 410.285±32.084µg/mL by TA and 459.126±38.629µg/mL by TV. When two-way ANOVA was applied it was also found to be significant with p value < 0.05. Overall, the isolated TS species can be rapidly and efficiently produced using the optimum conditions for effective management of CF with good plant growth promoter activty, thus serve as an eco-friendly approach for plant protection and yield increase, thereby economically useful for agricultural industry.


Antagonism, biocontrol, Colletotrichum falcatum, Trichoderma, plant protection.

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