Hypsizygous ulmaris  spent paddy straw and coir pith was enriched with beneficial microorganisms such as Azatobacter chroococcum, Bacillus megaterium and Trichoderma harzianum  individually and as consortia.  H. ulmaris spent coir pith with consortia of beneficial microorganisms along with sand and soil found to be better nursery mix compared to individual inoculation of  beneficial microorganisms for growth of tomato seedlings. Among the spent mushroom substrate spent mushroom coir pith supported the better growth and showed good dehydrogenase activity compare to spent mushroom paddy straw.