In vitro susceptibility testing of forty five isolated dermatophytes against four crude extracts of henna (Lawsonia inermis), handal (Citrullus colocynthis), indian fig (Opuntia ficus-indica) and pomegranate (Punica granatum) were evaluated. A minimum Inhibitory Concentration of the crude plant extracts required to inhibit the growth of
50 %  (MIC₅₀) and of 90 % (MIC₉₀)  of dermatophytes were estimated by a broth macrodilution method [National Committee for Clinical Laboratory Standards for filamentous fungi NCCLS, 2000 (M38-P)]. The results demonstrated that extract of pomegranate was  the highest activity as antifungal against for isolated dermatophytes. The mean of MIC₅₀ and MIC₉₀ were 0.8 to 1.6 mg/ml,  respectively. Among of the crude extracts, extract of prickly  pear was the least activity. M. canis and  T. rubrum were the more sensitivity to plant extracts whereas T. mentagrophytes  and T. verrucosum were the more resistant than other isolates. These results concluded that all crude extracts showed fungicidal activity against isolated dermatophytes. This study suggested that extracts of those plants could be used as a natural biological agents for a treatment of dermatophytosis.