ISSN: 0973-7510

E-ISSN: 2581-690X

DSVGK. Kaladhar1 , S. Harasreeramulu2, K. Vijaya Rachel1 and CH. Surekha1
1Department of Biochemistry and Bioinformatics, GITAM University, Visakhapatnam, India.
2Department of Biotechnology, Dr.V.S Krishna Govt. PG College, Visakhapatnam, India.
J Pure Appl Microbiol. 2009;3(2):759-767
© The Author(s). 2009
Received: 11/01/2009 | Accepted: 28/03/2009| Published: 31/10/2009
Abstract

Medicinal plants have been used virtually in all cultures as a source of medicine. The fresh leaves of in vivo grown and in vitro grown plants of Evolvulus alsinoides, Evolvulus nummularius, and Merremia tridentata were collected and examined for antimicrobial activity. Zone and turbidity methods are employed for antimicrobial assay. Methanolic extracts of the leaf parts of in vivo and in vitro grown cultures have exhibited antimicrobial activity. The extracts are found to be more effective against gram-positive bacteria and yeast (MIC of 0.05 to 0.25mg/ml) than gram-negative bacteria (MIC of 0.25 to 50 mg/ml). The extracts are found to be more effective against bacteria rather than filamentous fungi. The zone formation and MIC results from in vitro grown leaf extracts were less compared to the in vivo grown leaf extracts.

Keywords

In vitro, in vivo, Convolvulaceae, Antimicrobial activity, MIC

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