Evaluation of Antifungal Activity of Lactic Acid Bacteria against Aspergillus flavus

ISSN: 0973-7510

E-ISSN: 3187-3192

Evaluation of Antifungal Activity of Lactic Acid Bacteria against Aspergillus flavus

M. Ashwini¹ , Mahesh V. Kamble¹, Smita R. Babar¹, Shamrao Jahagirdar², K. S. Jagdeesh³ and J. A. Mulla⁴

¹AICRP on EAAI (Bioconversion Technology), Main Agriculture Research Station, University of Agricultural Sciences, Dharwad-580005, Karnataka, India.

²Department of Plant Pathology, UAS, Dharwad, India.

³Department of Agri. Microbiology, UAS, Dharwad, India.

⁴Department of Animal Science, UAS, Dharwad, India.

J. Pure Appl. Microbiol., 2015, 9 (4): 3187-3192.

Received: 12/03/2015 | Accepted: 26/04/2015 | Published: 31/12/2015

Abstract

Lactic acid bacteria (LAB) are considered as promising natural biological antagonists for mycotoxigenic fungi that contaminate various food commodities. A total 176 LAB isolated from different sources were screened against A. flavus by employing Dual culture assay method. Out of 176, two isolates viz., LS-36 and LS- 44 showed highest inhibition of the toxicogenic fungi under in vitro conditions as well as reduction of fungal mycelium under in vivo conditions. Based on our previous studies, 16S rRNA based molecular characterization of these two isolates showed sequence homology of 99 to 100% towards Lactobacillus brevis.

Keywords

Lactic Acid Bacteria, Aspergillus flavus, Antifungal activity, 16S rRNA.

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