ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ahmed R. Sofy1 , Akram A. Aboseidah2, El-Shahat El-Morsi2, Heba A. Azmy2 and Ahmed A. Hmed1
1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, 11884 Nasr City, Cairo, Egypt.
2Botany and Microbiology Department, Faculty of Science, Suez University, Suez, Egypt.
J. Pure Appl. Microbiol., 2020, 14 (1): 595-608 | Article Number: 6036
https://doi.org/10.22207/JPAM.14.1.62 | © The Author(s). 2020
Received: 12/01/2020 | Accepted: 21/03/2020 | Published: 02/04/2020
Abstract

The biggest problem in the world today is antimicrobial resistance, which undermines human health and increases morbidity and mortality associated with life-threatening serious diseases. Scientists from different fields are therefore examining plants for their antimicrobial use in the face of multidrug-resistant bacteria with a new eye. So, the aim of this research to find a new antibacterial from five aqueous extracts of Nigella sativa, Ziziphus spina-christi, Rosmarinus officinalis, Origanum majorana, and Allium sativum medicinal plants tested against the most multidrug-resistant bacterial isolates from clinical specimens which were Klebsiella pneumoniae (Gram-negative), Escherichia coli (Gram-negative), Pseudomonas aeruginosa (Gram-negative), Staphylococcus aureus (Gram-positive) and Methicillin-resistant Staphylococcus aureus (Gram-positive). Also, to investigate the antibiofilm activity of the most potent extract. From all tested plants, the total extract of Z. spina-christi appeared a strong antibacterial effect against all tested MDR-strains. Besides, its polyphenol fraction showed a stronger effect. Furthermore, the total extract MIC ranged between 3.125-12.5 mg/ml with MBC was 3.125-25 mg/ml against previous strains. While, polyphenol fraction MIC and MBC were about 0.312-1.25 mg/ml and 0.312-2.5 mg/ml, respectively. In contrast to the antibacterial strength of polyphenol fraction, the antibiofilm effect was weaker than the total extract antibiofilm effect, where at ½ MIC, the reduction of biofilm was about 78.18%, 81.9% and 99.48% against S. aureus, MRSA and P. aeruginosa, respectively. While, in the case of polyphenol fraction, the biofilm reduction effect against previous strains was 63.82%, 59.97%, and 87.95%, respectively.

Keywords

Herbal extracts, Medicinal plants, Antimicrobial agent, MDR-bacteria, MRSA.

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.