Present work deals with optimize the cultural condition and medium composition for enhancing the tannase production by Rhodotorula glutinis DB2 in submerged fermentation.  A tannase-producing read yeast strain R. glutinis DB2 was isolated from hydrolysable tannin solution (extracted from Rhizophora apiculate bark) that kept for a year at room temperature (30±2°C). This yeast strain showed ability to produced extracellular tannase significantly under submerged fermentation using tannic acid as an inducer. The rapid degradation of tannic acid and production of extracellular tannase was observed in the submerged fermentation medium. R. glutinis DB2 produced maximum extracellular tannase activity at the optimum bioprocess condition with 0.75 U/ mL and the yeast growth of 8.7 g/L.   These results indicate that an increment about 83% of tannase production was obtained compared to before optimization of cultural condition (0.41 U/ mL).