ISSN: 0973-7510

E-ISSN: 2581-690X

S. Uthra, R. Surjith, B.S. Harish and Kiran Babu Uppuluri

1Bioprospecting Laboratory, Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur – 613 401, India.
J. Pure Appl. Microbiol. 2014, 8(6):4637-4645
© The Author(s). 2014
Received: 26/07/2014 | Accepted: 14/09/2014 | Published: 31/12/2014

Oxytetracycline (OTC) is a macrolide broad spectrum antibiotic produced widely by microbial fermentation using Streptomyces Species. In the present study, pretreated sugar cane molasses (SCM), an industrial crop by-product was used as main substrate for the production of OTC from Streptomyces rimosus NCIM 2213. Four different pretreatment methods and their combinations were applied to SCM to remove the heavy metals and toxins. Sulfuric acid treated molasses was found effective based on the cell mass and OTC yield. The enhanced production of OTC was achieved by statistical optimization using Central composite design with four chosen variables. From the second order polynomial equation, the optimum OTC concentration was attained by 537.73 mg/l from S.rimosus in sulfuric acid treated sugar cane molasses. To the best of our knowledge this is the highest yield of OTC obtained in submerged fermentation process. The Spectral characterization of purified OTC by UV, FTIR, 1H NMR and 13C NMR confirmed that the structure is to be homologous to the standard sample. Purified fraction was also showed remarkable antimicrobial activity against various Gram positive and Gram negative organisms.


Oxytetracycline (OTC), Molasses, Pretreatment, Streptomyces rimosus, Optimization, Central Composite Design (CCD)

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.