Enhanced Production of Endochitinase from An Antarctic Bacterium, Sanguibacter antarcticus PAMC21702, in Pichia pastoris

ISSN: 0973-7510

E-ISSN: 2581-690X

Enhanced Production of Endochitinase from An Antarctic Bacterium, Sanguibacter antarcticus PAMC21702, in Pichia pastoris

Se Jong Han^1,2 , Sung Gu Lee^1,2, Heeyong Park¹, Hye Yeon Koh^1,3 and Joung Han Yim¹

¹Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, South Korea.

²Department of Polar Sciences, University of Science and Technology, Incheon 406-840, South Korea.

³Department of Applied Marine Biotechnology and Engineering, Gangneung-Wonju National University, Gangneung 210-702, South Korea.

J Pure Appl Microbiol. 2014;8(5):3531-3536

Received: 10/12/2013 | Accepted: 22/01/2014 | Published: 31/10/2014

Abstract

The recombinantendochitinase (rChi21702) from apsychrophilic Antarctic bacterium, Sanguibacter antarcticus(KCTC 13143, PAMC 21702),has been produced using Pichia pastorisexpression system. In this study, theoptimumfermentation temperature (20°C) and growth media pH (6) for Chi21702 production was determinedusing a2-stage fed-batch culturesystem.The maximal enzyme activity (178 U/l)of rChi21702obtained using a glycerol-methanol fed-batch culture system wasapproximately6-fold higher than that of unoptimized conditions. In addition, therChi21702 exhibitedcold-activeendochitinase activity and higher thermal stability than that of the wild-type Chi21702.This work allows forlarge-scale production ofrChi21702, which could be extended to further application studies using recombinant chtinases.

Keywords

Antarctica, Cold-active, Endochitinases, Fed-batch, Pichia pastoris

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