In the present study, we have investigated the Echis pyramidum crude venom induced oxidative stress and apoptosis after 2, 4 and 6 hrs in livers of rats injected with the [LD₅₀ and µ LD₅₀. Wistar rats were randomly divided into 3 groups, the control group was intrapretoneal (i.p.) injected with saline while, [LD₅₀ and µ LD₅₀ doses envenomed groups i.p. injected with venom at a dose of 0.6325 and 1.265 mg/kg body weight, respectively. Animals were sacrificed after 2, 4 and 6 hrs from the injection. Lipid peroxidation, nitric oxide and glutathione levels as oxidative markers were measured in liver homogenate. In addition, liver functions parameter and activity of catalase were determined. E. pyramidum crude venom increased lipid peroxidation and nitric oxide production in liver with concomitant reduction in glutathione, catalase, and total antioxidant capacity. These findings were associated with apoptosis induction in the liver as indicated with the increment in BAX expression. In addition, E. pyramidum crude venom caused hepatic injury as indicated by histopathological changes in the liver tissue with an elevation in total bilirubin, serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase. On the basis of the present results it can hypothesized that E. pyramidum crude venom is a potent toxins-mediated hepatotoxicity associated with apoptosis induction in the hepatic tisue.