ISSN: 0973-7510

E-ISSN: 2581-690X

Cui Ling-ling
1Department of Stomatology, the First Hospital Affiliated to Liaoning Medical College, Jinzhou – 121 001, China.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: April):283-288
© The Author(s). 2013
Received: 03/03/2013 | Accepted: 14/04/2013 | Published: 30/04/2013
Abstract

The aim of this study was to isolate and characteristics human dental pulp stem cells (hDPSCs) derived from human third molar pulp. Another aim was to verify the expression with dentin sialoprotein (DSPP), dentin sialoprotein (DSP), dentin matrixprotein 1 (DMP-1) and vimentin. For characterisation, proliferation capacity, phenotypic properties, and differentiation characteristics were utilised. Stem cells isolated from hNDP were analysed by flow cytometry and immunocytochemistry. Cell line were directionally differentiated towards adipogenic, osteogenic chondrogenic, myogenic and neurogenic lineages. The expression of DSPP,DSP,DMP-1and vimentin, as analyzed by Immunocytochemistry , increased after EMPs incubating for 5d. So exhibit multilineage differentiation properties. Thus, both EMPs are important during hDPSCs proliferation. During cytodifferentiation, EMPs are essential for the complete differentiation of odontoblasts by up-regulating the expression of DSPP, DSP, DMP-1 and vimentin. Stimulation of these cells by EMPs significantly increases alkaline phosphatase(ALP) activity. ALP activity was expressed as OD 405 nm/mg of protein A increase was observed in ALP activity of hDPSCs during the culturing of EMPs. According to the differentiation and proliferation potential of HDPSC was shown. A specific function for EMPs up-regulat in differentiation of hDPSCs was elucidated.

Keywords

Dental pulp stem cells, EMPs, DSPP, DSP , DMP-1, vimentin

Article Metrics

Article View: 0

Share This Article

© The Author(s) 2013. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.