ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
M.M.J. Taufiq and I. Darah
Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
J. Pure Appl. Microbiol., 2019, 13 (4): 2517-2531 | Article Number: 5895 | © The Author(s). 2019
Received: 06/10/2019 | Accepted: 19/12/2019 | Published: 28/12/2019

The effects of cultural conditions on the production of anti-MRSA activity of the endophytic fungal isolate, Lasiodiplodia pseudotheobromae IBRL OS-64 isolated from the leaf of a local medicinal herb, Ocimum sanctum Linn. was studied. The highest anti-MRSA activity of 50.0±0.1 U/mL and 1.46±0.1 g/L of fungal growth were obtained after incorporating all the improved cultural conditions which consisted of yeast extract sucrose broth supplemented with host plant extract, with initial medium pH of 6, 2 mycelial plugs of 4 days old seed culture, cultivation temperature of 30°C and cultivated for 16 days under static conditions without the presence of light. After improvement of cultural conditions, a significant increment of anti-MRSA activity of about 57.57% (2.4 folds) and a slight increment of fungal growth of about 8.15% (1.1 folds) were obtained compared to the cultural condition before improvement. Indeed the improvement of cultural conditions greatly affected the anti-MRSA activity and growth production by L. pseudotheobromae IBRL OS-64 isolate.


Endophytic fungus, Lasiodiplodia pseudotheobromae IBRL OS-64, Ocimum sanctum Linn, anti-MRSA activity, submerged fermentation.

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