ISSN: 0973-7510

E-ISSN: 2581-690X

O.O. Agarry , O.B. Olorunfemi, Y.A. Babarinde and F.C. Adetuyi
Department of Microbiology, Federal University of Technology, P.M.B. 704. Akure. Nigeria.
J Pure Appl Microbiol. 2007;1(1):17-20
© The Author(s). 2007
Received: 20/02/2007 | Accepted: 26/02/2007 | Published: 30/04/2007

Transgenic plants of cowpea (Vigna  unguiculata (L) Walp) were generated using Agrobacterium tumefaciens. The antibiotics that eliminated the Agrobacterium tumefaciens totally were 200mg/l ampicillin and 500mg/l cefotaxime. The co-cultivated embryos muddled with Agrobacterium were cultured on different concentration of Carbenicillin, Augmentin, Ampicillin and Cefotaxime. The surviving shoots were transferred every ten days to ensure cleaning and contact with the medium. At each transfer the number of surviving shoot decreased due to shock of Agrobacterium contamination until it stabilized. Those plated on 200mg/l Ampicillin and Cefotaxime eventually produced new green healthier auxiliary shoots which could be transferred to the soil.


Transgenic plants, Antibiotics, Agrobacterium tumefaciens

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© The Author(s) 2007. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.