Most of the Pleurotus sp. can use variety of substrate materials than any other mushroom. In this study, effect of dried mango leaves aqueous extract (MLAE) upon radial mycelial growth of Pleurotus sajor–caju and Pleurotus florida was evaluated on eight different waste substrate materials which included, mango leaves, wheat straw, sugarcane bagasse, waste cotton, waste paper, wood shavings, paddy straw, and coconut coir. Best growth was observed on dried mango leaves. Radial mycelial growth was significantly increased when sugarcane bagasse and coconut coir were supplemented with the extract of dried mango leaves. Notable increase in growth was observed in case of paddy straw and waste paper upon supplementation. Mango leaves extract based agar medium was found to be better than Sabouraud’s dextrose agar (SDA) medium.