Production of extracellular a- amylase enzyme by a filamentous fungus, Aspergillus niger was studied in rice submerged fermentation. The potential strain was successfully mutated by ultraviolet (UV) and ethyl methane sulphonate (EMS). High level of a- amylase activity was obtained by the mutant strains UV-3 and EMS-2. These strains exhibited 1.44 and 2.19 times increased production of a- amylase respectively than the wild strain, which reveals the potential use of mutant strains in the industries.
Aspergillus niger, a- amylase, EMS, UV, Mutation, Ascomycetous fungi
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