ISSN: 0973-7510

E-ISSN: 2581-690X

Ivana Cabarkapa1 , Marija Skrinjar2, Jovanka Levic1, Nevena Blagojev2, Bojana Kokic1 and Ljiljana Suvajdzic3
1University of Novi Sad, Institute of Food Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
2University of Novi Sad, Faculty of Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
3University of Novi Sad, Faculty of Medicine, Department of Pharmacy, Novi Sad, Serbia.
J Pure Appl Microbiol. 2015;9(Spl. Edn. 1):123-129
© The Author(s). 2015
Received: 23/02/2015 | Accepted: 09/04/2015 | Published: 31/05/2015
Abstract

Bacteria within a biofilm have a distinct phenotype from planktonic cells and generally show higher tolerance to antimicrobial agents, such as disinfectant treatments and antibiotics. Due to the increased tolerance against antimicrobial treatments, biofilms are hard to eradicate and they cause all kinds of problems in medical and industrial settings. Therefore, the purpose of this study was to examine the influence of Thymus vulgaris essential oil on the initial adhesion and preformed biofilm of Salmonella Enteritidis. Results for antimicrobial activity of Th. vulgaris essential oil obtained using broth microdilution method against S. Enteritidis were identical for all tested isolates (MIC/MFC=0.156/0.3125 µL/mL). In order to understand the anti-biofilm action of essential oil (EO), its effect was tested on both the initial cell attachment by planktonic cells as well as on preformed biofilms. Obtained results indicated that the effect of Th. vulgaris essential oil on initial cell attachment of tested isolates SE4, SE5, SE7 and SE9 was dosage dependent manner, although even at 0.5×MIC biomass attachment was reduced by 74.2%, 77.7%, 73.6% and 70.1%, respectively. Fairly high, but not complete, inhibition of cell attachment was achieved using 1×MIC of this EO. Using 1×MIC, biomass attachment of tested isolates SE4, SE5, SE7 and SE9 was reduced for 91.4%, 90.2%, 90.6% and 88.4%, respectively. When the same EO was tested against a preformed biofilm, its inhibitory effect was reduced greatly.

Keywords

Salmonella Enteritidis, biofilm, Thymus

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© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.