ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Hayder Hamzah Ibrahim1 , Naeem  Rahman aljeburi 2, Muna Sabbar Jebar1, Mustafa Ali Gayeb1,  Saher Saleh Mahdi1, Hadeer Ali Kudder1, and Marwa Majed Naseer1
1Department of Medical Analysis, Babylon Technical Institute, Al-Furat Al-Awsat Technical University, Babylon, Iraq.
2Hilla General teaching Hospital, Babylon, Iraq.
J. Pure Appl. Microbiol., 2018, 12 (4): 2241-2244 | Article Number: 5324
Received: 21/09/2018 | Accepted: 04/11/2018 | Published: 29/12/2018
Abstract

The purpose of the present study is to evaluate the effect sub minimum inhibitory concentration (MIC) of imipenem, amikacin and cefixime on growth and swarming of Proteus mirabilis. 10 clinical isolates of Proteus mirabilis were isolated from patients suffering from urinary tract infection. 3 types of antibiotics include imipenum, amikacin and cefixime were used in present study to detect their ability to inhibition the swarming of P. mirabilis. Each isolate were inoculated on a series of nutrient agar containing different concentrations (60, 30, 15, 7.5, 3.75, 1.8 and 0.9 mg/ml) of imipenem, cefixime and amikacin. All these were incubated over night at 37°C, after that the cultivated bacterial growth examined to determine the effect of each antibiotic on growth and swarming of P. mirabilis. The results showed that the 0.93 mg/ml of imipenem have ability to inhibit the swarming of all isolates (100%) and it have not any effect on growth (bacterial growth 100%) whereas the same concentration of cefixime inhibit 90% of isolate and also have not any effect on growth (bacterial growth 100%). The results of amikacin showed that the 0.93 mg/ml of antibiotic inhibit the swarming 50% of isolate and the bacterial growth was 100%. Imipenem have good inhibiter effect on Proteus mirabilis swarming at concentration of 0.93 mg/ml and we suggest that we can use this antibiotic to prepare cultural media to separate mixed culture contain P. mirabilis.

Keywords

Urinary tract infection, Proteus, swarming, Imipenem, Amikacin, Cefixime.

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