Biogas, a renewable source of energy which is also environmentally friendly, is generated via anaerobic digestion of biomass wastes (animal dung, plant residues, food waste, municipal solid waste etc). The effect of seasonal variation on the rate of biogas production from different food waste was carried out. The food waste used for the study are cooked food, wasted tomatoes, fruit waste( wasted bananas), vegetable cut outs (fenugreek) and waste from candy preparing industry. The substrates were monitored for biogas production; the parameters analyzed were pH, alkalinity, chemical oxygen demand, proteins and carbohydrates. The study was carried for summer season (March, April and May) and winter season (November, December and January). The pH variation in all the substrate as well the chemical oxygen demand reduction was observed. The maximum chemical oxygen demand reduction was observed in industrial waste in the summer season. The study confirmed that maximum biogas production occurs in summer season due to thermophilic reaction.