Thirty-nine phosphate solubilizing bacteria (PSB) were isolated from the rhizosphere of green gram and mustard by using Pikovskaya medium containing tricalcium phosphate. All the PSB showed a large variation in P- solubilization on soild as well as in liquid media. Most of the isolates fell in <100% class of P- solubilization, while in liquid media 50-110 µg/ml. No correlation was established between P solubilization on solid and liquid media. The selected isolates 4GRP, 25MRP, 27MRP, 28MRP, 33MRP and 34MRP showed P- solubilization 168.3 µg/ml, 260.8 µg/ml, 255.4 µg/ml, 261.6 µg/ml, 220.5 µg/ml and 175.6 µg/ml respectively. The pH of the medium was decreased by PSB during the P-solubilization, maximum decrease in pH was found with 25 MRP. Under pot house conditions at 60 DAS in green gram and mustard, maximum plant dry biomass was recorded with 25MRP with URP followed by 33MRP with URP. In mustard maximum P uptake was observed in 25MRP with URP (284%) followed by 4GRP with URP (143%) at 60 DAS. In green gram maximum P uptake was observed in 25MRP with URP (224%) followed by 33MRP with URP (182%) at 60 DAS.
Phosphate solubilizing bacteria (PSB) – Green gram, mustard, Plant growth
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