ISSN: 0973-7510

E-ISSN: 2581-690X

Meenu Rathi1, Deepak Kumar Malik2, Pankaj Bhatia3, Mukesh Kumar3, Aarti Dahiya1 and Baljit Singh Dahiya1
1Department of Botany, Janta Vedic College, Baraut – 250 611, India.
2Deapartment of Biotechnology Engineering, UIET, Kurukshetra University, Kurukshetra-132119, India.
3Deapartment of Biotechnology Engineering, Ambala College of Engineering and Applied Research, Ambala – 133 101, India.
J Pure Appl Microbiol. 2009;3(1):125-133
© The Author(s). 2009
Received: 08/09/2008 | Accepted: 03/12/2008| Published: 30/04/2009

Thirty-nine phosphate solubilizing bacteria (PSB) were isolated from the rhizosphere of green gram and mustard by using Pikovskaya medium containing tricalcium phosphate. All the PSB showed a large variation in P- solubilization on soild as well as in liquid media. Most of the isolates fell in <100% class of P- solubilization, while in liquid media 50-110 µg/ml. No correlation was established between P solubilization on solid and liquid media. The selected isolates 4GRP, 25MRP, 27MRP, 28MRP, 33MRP and 34MRP showed P- solubilization 168.3 µg/ml, 260.8 µg/ml, 255.4 µg/ml, 261.6 µg/ml, 220.5 µg/ml and 175.6 µg/ml respectively. The pH of the medium was decreased by PSB during the P-solubilization, maximum decrease in pH was found with 25 MRP. Under pot house conditions at 60 DAS in green gram and mustard, maximum plant dry biomass was recorded with 25MRP with URP followed by 33MRP with URP.  In mustard maximum P uptake was observed in 25MRP with URP (284%) followed by 4GRP with URP (143%) at 60 DAS. In green gram maximum P uptake was observed in 25MRP with URP (224%) followed by 33MRP with URP (182%) at 60 DAS.


Phosphate solubilizing bacteria (PSB) – Green gram, mustard, Plant growth

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© The Author(s) 2009. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.