Effect of Nitrogen Sources and C:N Ratio on Production of Manganese Peroxidase using Phanerochaete chrysosporium NCIM 1197

ISSN: 0973-7510

E-ISSN: 2581-690X

Effect of Nitrogen Sources and C:N Ratio on Production of Manganese Peroxidase using Phanerochaete chrysosporium NCIM 1197

Alok Kumar Varshney^1,2, Medicherla Krishna Mohan^1,2, Ambarish Sharan Vidyarthi², Vinod Kumar Nigam² and Purnendu Ghosh¹

¹Department of Biotechnology, Birla Institute of Scientific Research, Jaipur – 302001, Rajasthan, India.

²Department of Biotechnology, Birla Institute of Technology, Mesra 835215, Ranchi, Jharkhand, India.

J Pure Appl Microbiol. 2014;8(1):481-487

Received: 05/06/2013 | Accepted: 19/07/2013 | Published: 28/02/2014

Abstract

A lignin degrading enzyme, manganese peroxidase has detected in culture supernatants of Phanerochaete chrysosporium NCIM 1197 grown under various nitrogen sources. The present work was carried out to determine the effect of variable concentration of ammonium tartrate, ammonium chloride and urea on MnP production. Highest level of manganese peroxidase activity, (118.60 ± 2.26 U/ml) was observed in the production medium supplemented with urea (0.0108 mM) and glucose (55.55 mM). A very high C:N ratio in the medium favours the MnP production. The production of the MnP enzyme is a major demand in environmental biotechnology, so outcome of this study can help in scale-up of the enzyme.

Keywords

Ligninolytic enzyme, Manganese peroxidase, Nitrogen source, Phanerochaete chrysosporium, White rot fungi

Download PDF

Article Metrics

Article View: 952

JPAM.8.1.59 (233 downloads)

Share This Article

© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.