Total 30 extreme halophilic isolates were obtained from salt, mud and water samples collected from little Rann of Kutch, Gujarat, India after enrichment. Morphologically all the isolates were Gram’s positive or Gram’s variable and non-capsulated. Primary screening of lipase producer was performed on tributyrene agar. On the basis of zone index, Ku-20 was found to be a potential lipase producer, selected for further study in liquid media. Organism was identified on the basis of 16s r-RNA sequencing as Halomonas salina. Organism was able to secrete maximum lipase after 264 hours of incubation, 10-15% NaCl, pH 7, 30°C temperature and 2% tributyrene concentration.
Extreme halophiles, Halomonas salina, Lipases, Rann of Kutch
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