ISSN: 0973-7510

E-ISSN: 2581-690X

Mahwish Salman1 , Muhammad Shahid†1, Amer Jamil1 and Sajjad Ur Rahman2
1Department of Biochemistry, University of Agriculture, Faisalabad – 38040, Pakistan.
2Institute of Microbiology, University of Agriculture, Faisalabad – 38040, Pakistan.
J Pure Appl Microbiol. 2014;8(5):3721-3728
© The Author(s). 2014
Received: 10/08/2014 | Accepted: 23/09/2014 | Published: 31/10/2014
Abstract

Lactobacillus acidophilus produced a small bacteriocin of estimated molecular mass of 6.5 kDa. This bacteriocin exhibited strong antibiofilm activity against Gram positive food borne pathogen, Bacillus cereus. In addition, this antibacterial peptide also exhibited strong antagonistic activity against other Gram positive bacteria, Staphylococcus aureus and Bacillus subtilis. In sequential bacteriocin purification steps, the salt precipitated bacteriocin extract of L. acidophilus displayed 50.74% bacterial growth inhibition (INH%) in 25% v/v bacteriocin treatment. Mean while, the ion exchange and gel filtration chromatography extracts displayed 58.29% and 60.20% bacterial growth inhibition. Whereas in 50% v/v bacteriocin treatment, the salt precipitated, ion exchange and gel filtration chromatography extract of bacteriocin displayed bacterial growth inhibition as 72.79%, 87.12% and 96.42% respectively. The microscopic observation showed that there was an increased biofilm inhibition activity in highly purified bacteriocin extract that confirmed significant inhibition activity of bacteriocin against the formation of biofilms.

Keywords

Lactobacillus acidophilus, Bacillus cereus, Biofilm, Bacteriocin, Column chromatography

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