ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Mohammed M. Aljeldah1, Hosam El-Sayyad2* , Nasreldin Elhadi3 and Ali A. Rabaan4
1Department of Medical Laboratory Technology, College of Applied Medical Sciences, University of Hafr Albaten, Hafr Albaten Saudi Arabia.
2Middle Eastern Regional Radioisotope Centre for the Arab Countries, Dokki, Giza, Egypt. (Al-Ghad International College for Applied Medical Sciences Dammam, Kingdom of Saudi Arabia).
3Department of Clinical Laboratory Science, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, P.O. Box 2435, 31441 Dammam, Kingdom of Saudi Arabia.
4Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia.
J Pure Appl Microbiol, 2019, 13 (2): 779-788 | Article Number: 5351
Received: 18/02/2019| Accepted: 20/04/2019 | Published: 24/06/2019

This study aimed to investigate the effects of different doses (25, 50, 75, 100, 125, 150, 200, 250, 500, 1000 and 2000 Gy) of gamma radiation on spore germination in Penicillium chrysogenum and on the production and efficacy of penicillin in this fungus. A dose of 200 Gy of gamma radiation increased spore germination by 70.2% after 20 hours of incubation compared to control cultures. It was also found that penicillin yield increased by 95.2% at this dose of gamma radiation. The efficacy of penicillin was remarkably enhanced, resulting in a 99.6% reduction in the total count of Staphylococcus aureus treated with penicillin from irradiated P. chrysogenum compared with that of S. aureus treated with commercially available and purified penicillin from nonirradiated fungi. The results obtained highlighted the potential of this technology for production of penicillin.


Penicillium chrysogenum, penicillin, gamma radiation.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.