The several fungi are edible mushrooms and are saprophytic basidiomycetes, which have been successfully cultivated at commercial level worldwide using lignocellulose wastes as substrates for their cultivation. In the present investigation that were the studies of effect of different culture media, pH and temperature on mycelia growth of strains (S-79, A-15 and Delta) of A. bisporus. Five culture media were tested for the radial growth and measured the mycelia growth on PDA  of three strains S-79, A-15 and Delta were obtained fast and full growth (9 cm) completed in 8, 10 and 12 days respectively. The effect of different pH were tested for the radial growth and measured the mycelial growth on 9 pH  of all three strains S-79, A-15 and Delta were obtained fast and full growth (9 cm) completed in 12, 12 and 14 days respectively. The growth of mycelial in 8pH was good performance in all strains of A. bisporus followed by 7 pH. Mycelial growth was much slow on 6 pH and full growth completed in 18 days. The effect of different temperature was evaluated on mycelia growth of strains of A. bisporus. Mycelial growth of all three strains on 25°C was obtained fast and full growth (9 cm) completed in 8, 10 and 14 days respectively, followed by growth of mycelial at 20ºC and 30°C were similar performance in all strains of A. bisporus and the mycelial growth was much slow on 15°C and full growth completed in 18 days.